SOD1 and amyotrophic lateral sclerosis: To address this issue, we first analyzed reactive oxygen species (ROS) and lipid peroxidation levels to monitor oxidative stress; secondly, we used the Seahorse Bioscience XF Analyzer to monitor oxygen consumption rate (OCR) and the rate of extracellular acidification (ECAR), and finally we determined changes in metabolism in lymphoblasts from healthy controls and sALS and SOD1-ALS mutation carriers.