In the primary tumours, the SNS allele frequencies ranged between less than 5% to about 30%: the four SNSs that were identified in all organoids had allele frequencies of about 30%; whereas the allele frequencies of the remaining SNSs were lower with the interesting exception of the SNSs that targeted the Ociad2 and Vmn1r119 genes, which had allele frequencies close to 30%, even though they were identified in only 3 out of the 7 organoids (Figure S10). This evidence concerns the gene OCIAD2 and neoplasm.