To investigate the potential effects of CHPF on malignant HCC cell phenotypes, we measured the CHPF protein levels in seven HCC cell lines and found that HA22T, HCC36, and Hepa1-6 expressed lower CHPF levels, whereas Hep3B and HA59T expressed higher CHPF levels (Figure 2A). Here, CHPF is linked to hepatocellular carcinoma.