Our results showed that the increased Foxp3+ Treg cells, along with the scanty CD8+ T cells, were associated with MitoX-induced autophagy-dependent PAI-1 secretion (Figure 4, Figure 5 and Figure 6), suggesting that tumor autophagy modulates the tumor immune microenvironment in response to chemotherapy via the secretory pathway. This evidence concerns the gene CD8A and neoplasm.