Among the 12 candidate pathogenic variants, only the pathogenic variant chr3:126066633A>T (GRCh37.p13: GenBank accession no. NC_000003.11), equivalent to chr3:126293291A>T (GRCh38.p13: GenBank accession no. NC_000003.12) or NM_014079.4: c.685A>T; p.(Lys229*) in the KLF15 gene, was verified by Sanger sequencing with the primers shown in Table 2, and co-segregated with AF in the whole family. The gene discussed is KLF15; the disease is atrial fibrillation.