According to our results, the co-activation via CD180 and BCR and anti-CD180 stimulation on its own resulted in increased phosphorylation of NF-κB in dcSSc B cells, but the level of phosphorylation was not higher in dcSSc than in HC, even though the CD180 MFI of naive B cells was lower in dcSSc than in HC and naive B cells are increased in SSc compared to HC. Here, NFKB1 is linked to systemic sclerosis.