Neither TRAIL nor AD-O51.4 showed toxic activity against the B-16 cell line (melanoma), whereas AD-O51.4 exhibited low toxicity toward mIMCD3 (kidney inner medullary collecting duct cells) and CT-26 (colon carcinoma) cells indicating that human ligand proteins to not interact or bind loosely with mouse TRAIL receptor. Here, TNFSF10 is linked to melanoma.