However, it was not possible to determine these in our 1H NMR plasma profiles since firstly, any sharp singlet resonances arising from such low-molecular-mass N-acetylsugar-containing saccharide species will be largely overlapped by the much higher intensity and broader ‘acute-phase’ glycoprotein signals, and these interferants are themselves overlapped by the similarly broad lipoprotein-associated TAG-CH2-CH=CH- function signal, as indeed they were in an NMR-based metabolomics study focused on evaluations of the metabolic profiles of NPC1 disease patients [20]. This evidence concerns the gene ART4 and Niemann-Pick disease, type C1.