To verify whether c10orf118 gene expression was accompanied to increased protein level, evaluation of the protein both in cell lysates and CM of cancer cell lines was assessed by means of an anti-c10orf118 antibody (HPA018019, Sigma Aldrich, Milano, Italy), directed against a 131-residue sequence within the central portion of the protein (Figure 2A). The gene discussed is CCDC186; the disease is cancer.