The overexpression of transcription factor 21 was found to upregulate qHSC marker genes (Gfap and Ngfr) and to downregulate profibrotic genes (Pdgfrb, Acta2, and Col1a1) in vitro, and it was found to further reduce Acta2 and Col1a1 expression in mice with CCl4– or methionine/choline-deficient diet-induced liver fibrosis, accompanied by the regression of fibrosis and steatohepatitis [128]. The gene discussed is ACTA2; the disease is Hepatic fibrosis.