In addition, we found that berberine could impair mitochondrial function by inhibiting mitochondrial protein, and GTPase ERAL1 as well as mitochondrial ribosomal proteins including MRPL11, 15, 30, 37, 40, and 52 have potential to serve as candidate targets of berberine in colon cancer cells. This evidence concerns the gene ERAL1 and colonic neoplasm.