SUMO2 and infection: For the system-wide identification of SUMOylation changes to target proteins, HepaRG cells stably expressing HA-His-SUMO2 were grown in SILAC medium, infected with HSV-1 for 12 h, and both total protein and Ni-NTA-purified proteins were analyzed to determine infection-induced changes to total protein abundance and SUMOylated proteins, respectively.