To identify possible the antibodies with the best specificity and sensitivity, Western blot assay method was used to analyze our home-made chicken antibody and two polyclonal rabbit antibodies from commercial sources for their abilities to recognize the full size huMETCAM/MUC18 protein in the whole cell lysates from various prostate cancer cell lines and the respective recombinant proteins that contain different epitopes. Here, MCAM is linked to Familial prostate cancer.