In the current study, untreated PCOS rats had ~10 mmHg increase in MAP accompanied by upregulation of intrarenal angiotensinogen mRNA expression as well as upregulation of ACE mRNA and protein compared to untreated controls, suggesting that a dysregulated intrarenal RAS is at least partially responsible for the elevated BP observed in PCOS model. This evidence concerns the gene ACE and polycystic ovary syndrome.