The combination of TGF-β1 and angiopoietin-2 level could improve the prognostic power to determine severe CCA stage in patients as shown in Figure 5E, view Supplementary Materials; Table S3 (32% sensitivity and 100% specificity, p = 0.002, AUC = 0.842, YI = 0.002). Here, ANGPT2 is linked to cholangiocarcinoma.