The best cut-off values that distinguished the CCA group from the non-CCA group (Figure 3A–E; right), of each protein were S100A9 (87.11 ng/mL), MUC5AC (90.51 ng/mL), TGF-β1 (39.98 ng/mL) angiopoietin-2 (1008 pg/mL), and CA19-9 (37.39 U/mL). This evidence concerns the gene S100A9 and cholangiocarcinoma.