TGFB1 and cholangiocarcinoma: For normal vs. CCA discrimination (Figure 3A–E; left), each cut-off values of S100A9 (197.9 ng/mL), MUC5AC (104.6 ng/mL), TGF-β1 (33.42 ng/mL), angiopoietin-2 (2422 pg/mL), and CA19-9 (23.34 U/mL) were generated from AUC analyses.