To distinguish the non-CCA group from the normal group, the resulting DT model, DT II with a hierarchical structure of angiopoietin-2, TGF-β1, and S100A9, was shown in Figure 6B. All non-CCA cases were discriminated against by serum angiopoietin-2 values < 1312 pg/mL in the control group (correctly classified 83.33% in the training dataset). Here, TGFB1 is linked to cholangiocarcinoma.