Results of the crude and adjusted OR of MUC5AC, angiopoietin-2, and CA19-9 were 2.79 (p = 0.027), 3.26 (p = 0.016), 4.33 (p = 0.019), 4.78 (p = 0.015), 11 (p < 0.0001), 12.7 (p < 0.0001), and respectively showed that they can differentiate CCA from non-CCA patients. This evidence concerns the gene MUC5AC and cholangiocarcinoma.