Analysis showed that the positive detection rate of PBC was 56% in the combined detection of anti-Sp140, anti-Sp100, and anti-PML antibody, compared with 27% in single anti-Sp140, 40% in single anti-Sp100, and 31% in single anti-PML antibody detection; thus, the diagnosis rate was increased by about 20%, and the difference was statistically significant, p < 0.05 (Table 2). The gene discussed is SP140; the disease is primary biliary cholangitis.