SP100 and primary biliary cholangitis: Positive detection rate of PBC AMA negative was 65% in the combined detection of anti-Sp140, anti-Sp100, and anti-PML antibody, compared with 41% in single anti-Sp140, 29% in anti-Sp100, and 35% in single anti-PML antibody detection in this group; thus, the diagnosis rate was increased about 25–30%, and the difference was statistically significant primarily for anti-Sp100 antibody, p = 0.038.