For validation purposes, additional house-keeping genes (HPRT1 and beta-actin (ACTB)) were added, based on the sequencing results that showed no change following IPF-CM exposure (fold change (FC) 0.98, p = 0.95 and 1.02, p = 0.69, respectively). This evidence concerns the gene HPRT1 and idiopathic pulmonary fibrosis.