We applied this technique to NSCLC cells adapted to grow in the presence of EGFR tyrosine kinase inhibitors as a cellular model of acquired drug resistance and found a constitutive activation of Akt in a subline of the NSCLC cell line HCC4006 adapted to the EGFR tyrosine kinase inhibitor erlotinib (HCC4006rErlo0.5) [7]. Here, EGFR is linked to non-small cell lung carcinoma.