Remarkably, TCOF1 mutations identified in TCS patients can affect amino acids involved in the interactions with Pol I, NOP56, UBF, Nopp144, and TOPBP1, or phosphorylation sites (Table S1), crucial for treacle functioning in ribosome biogenesis and DDR, suggesting another possible mechanism of treacle dysfunction in TCS. The gene discussed is TCOF1; the disease is Treacher-Collins syndrome.