IL13 and familial pancreatic carcinoma: IL-13 protein has also been detected in both total cell lysates and conditioned medium of COLO-357, MIA PaCa-2, PANC-1 ASPC-1, Capan-1, and T3M4 cells by ELISA, while the presence of IL-13-mRNA transcript was examined by Northern blotting [93], indicating that pancreatic cancer cells can produce and also secrete IL-13 to exert autocrine and paracrine effects.