Expression of endogenous IL-4 has also been determined in total cell lysates from pancreatic cell lines COLO-357, MIA PaCa-2, PANC-1, ASPC-1, Capan-1, and T3M4 on the protein level by ELISA, and on the mRNA level by real-time PCR analysis [54], which points to possible autocrine and paracrine actions in pancreatic cancer. The gene discussed is IL4; the disease is pancreatic neoplasm.