As shown in Figure 2D and Figure S1B, Annexin-V-PI double staining showed that the apoptotic marker AnnexinV/PI positive population was slightly but not significantly increased by 13R,20-diHDHA treatment of breast cancer cells, from 0.6% in the control group to 2.9% in the 40 μM 13R,20-diHDHA-treated group. This evidence concerns the gene ANXA5 and breast carcinoma.