Consistent with previous findings suggesting GRHL3 as a key regulator in urothelial differentiation, this study shows that GRHL3 is highly expressed in differentiated epithelial cells of normal human urothelium and well-differentiated RT4 bladder cancer cells, but its expression is reduced in RT112 cells and lost in the anaplastic bladder cancer cell line T24. Here, GRHL3 is linked to urinary bladder cancer.