To confirm the robustness and sensitivity of the expression changes observed in our proteomic data sets, expression levels of six DEPs (ROCK1, ROCK2, OGDH, GLS, KPNA2, and FAM49B), already known to be implicated in the pro-proliferative and apoptosis-resistant phenotype of PAH-PASMC or cancer cells [6,20,26,27], were measured by Western blotting in six different PAH-PASMC lines (including the four used in our unbiased proteomic approach). This evidence concerns the gene ROCK2 and pulmonary arterial hypertension.