STX2 and thrombotic microangiopathy: Adult rats i.p. injected with culture supernatant from recombinant E. coli expressing Stx2 (sStx2) can develop watery diarrhea, thrombocytopenia, hemolytic anemia, glomerular thrombotic microangiopathy, and tubular injury, which may result from the combined effect of Stx2 and other bacterial factors in the culture supernatant [84].