Case #40, for which the calibrated max-score was 0.31 for diffuse midline glioma (DMG) H3K27 mutant, clearly clustered with the tumors of the CNS neuroblastoma with FOXR2 activation (CNS NB-FOXR2) methylation class, which was totally consistent with the clinical and histopathological findings, and corresponded to the diagnosis suggested by the experts at the national case conference (Figure 3B, Table S1). This evidence concerns the gene FOXR2 and diffuse midline glioma.