To further investigate whether the use of circulating methylated CCDC181, GCM2 and ITPRIPL1 biomarkers could improve the detection of breast cancers, we combined the ultrasonography BI-RADS (3 and 4a) and circulating methylated CCDC181, GCM2 and ITPRIPL1 levels. The gene discussed is GCM2; the disease is breast cancer.