As shown in Figure 5a,b, the expression of pro-apoptotic proteins in HCC cells increased after being treated with compound 1 for 72 h, such as Cytochrome c, cleaved Caspase-9 and -3, cleaved PARP, BAX and Bad, while the expression of Bcl-2 significantly decreased. Here, CASP9 is linked to hepatocellular carcinoma.