To explore whether endogenous ANO1 expression correlates with the levels of selected proteins involved in mitochondrial apoptotic pathway, we studied by immunoblotting their expression patterns in five UT-SCC and two GIST cell lines with high ANO1 expression (UT-SCC-8, UT-SCC-14, UT-SCC-87, GIST48, GIST-T1), as well as two UT-SCC cell lines with low ANO1 expression (UT-SCC-11 and UT-SCC-95). This evidence concerns the gene ANO1 and gastrointestinal stromal tumor.