To elucidate in an unbiased fashion the role of ANO1 in the cancerous properties of the ANO1HIGH UT-SCC cell lines, we performed RNA sequencing from ANO1 silenced cell samples (shANO1) and their control counterparts (shScramble), cultured in three-dimensional collagen type I, which better recapitulates the tumor microenvironment [46]. Here, ANO1 is linked to neoplasm.