PROS1 and infection: Transfection of the wild-type CrPV and CrPV-aNCV containing the full-length aNCV IGR RNA resulted in expression of the CrPV non-structural protein 1A and the structural protein VP2 at 120 h post-transfection (h.p.i) (Figure 6C), which from previous experience is indicative of productive infection [30].