PLOD1 and mastitis: In fact, it is well known that the routine microbiological test applied for the diagnosis of mastitis fail to detect Leptospira. Therefore, during routine practice, it could be difficult to identify lactating animals infected by LH, although the excretion of LH in infected cow’s milk has been reported [10,32] and it may represent a zoonotic risk for human health [2,9,33,34].