Furthermore, neovascularization and increase in vegfa and cyclin D1 expression were observed in 85% of the embryos with NO production, and the addition of a NO scavenger (CPTIO) reduced vegfa and cyclin D1 expression as well as the number of endothelial cells, suggesting that the reduction in NO levels by nitric oxide scavenging could be an efficient approach for the treatment of glioma. This evidence concerns the gene CCND1 and glioma.