To examine the effects of Rac proteins on GBM progression in vivo, Lai et al. injected GFP-labeled U373- tumor sphere cells (GBM stem-like cells) in the yolk sac of 2 dpf Tg(kdr:mCherry) embryos with labeled endothelial cells, harboring control scramble short haipin (shRNA), Rac shRNAs (shRacs) and Rac 1–3 cDNAs, to silence or overexpress Rac proteins respectively [158]. The gene discussed is AKT1; the disease is neoplasm.