NT-pro-BNP was reported to be significantly higher in cats with heart disease in all studies, and some of them identified an optimal NT-pro-BNP cut-off value for the identification of occult cardiomyopathy with high sensitivity and specificity, using both traditional quantitative ELISA methods (first and second generation) and SNAP ELISA methods: between 100 and 122 pmol/L [109,110,111,112,115,117,118,119]. This evidence concerns the gene NPPB and heart disorder.