In the present STAM mouse NASH model, overexpression of the novel protein, CACHD1 was first recognized in HCCs of 18-week-old mice with the help of proteome analysis, and thereafter CACHD1+ foci and tumor formation was immunohistochemically confirmed in the livers of 10- and 18-week-old STAM mice. This evidence concerns the gene CACHD1 and metabolic dysfunction-associated steatohepatitis.