It was found that significantly different KEGG pathways predicted by PICRUSt between trilobatin administration (TRL, TRM, and TRH) and BC groups were mainly involved in lipid metabolism (steroid biosynthesis, steroid hormone biosynthesis), amino acid metabolism (D-arginine and D-ornithine metabolism), other secondary metabolites (flavonoid biosynthesis), terpenoid and polyketide (carotenoid biosynthesis), and cofactor and vitamin (retinol metabolism). The gene discussed is TRH; the disease is breast cancer.