In one study, tumor-infiltrating, TIM-3-expressing CD4+ T cells exhibited an impaired capacity to produce IFN-γ and interleukin(IL)-2, but expressed higher levels of CD25 and forkhead box protein 3 (Foxp3) than levels of peripheral blood and non-TILs, suggesting that the TIL expression of TIM-3 contributes to the formation of the immunosuppressive TME [40]. This evidence concerns the gene IFNG and neoplasm.