In vitro encounter with HER2+ SKOV3 ovarian carcinoma cells (Supplementary Figure S2C) induced Ifng and Il2 release from all four HER2-specific CAR T cell designs regardless of co-stimulatory moiety, but designs using 4-1BB co-stimulation also induced lower cytokine levels as in CAR T cells targeting L1CAM (Figure 4B). The gene discussed is ERBB2; the disease is ovarian carcinoma.