Furthermore, two-sample Kolmogorov–Smirnov (KS) tests revealed statistically significant increases (siUSP22-2, siUSP22-3) and a decrease (siUSP22-Pool) in cumulative nuclear area frequency distributions relative to siControl (Figure 4D; Table S5) that are consistent with reduced USP22 expression inducing CIN. Here, USP22 is linked to cervical squamous intraepithelial neoplasia.