We also selected NSCLC cell lines with high (H226B) or low (H1299) levels of IGFBP-3 expression [29] and established their counterparts (H226B-shBP3 and H1299-BP3) by stable transfection with shRNAs or by forced overexpression of IGFBP-3 (Figure 1B). The gene discussed is IGFBP3; the disease is non-small cell lung carcinoma.