In this present study, we investigated whether RF irradiation can mediate the expressions of important TRPV1- and TRPV1-mediated neuropeptides, including CGRP, PACAP, and VIP, which are active in the skin of mice with UVB-exposure rosacea (in vivo) and UVB- or heat-treated sensory neuronal cells (in vitro). This evidence concerns the gene TRPV1 and rosacea.