We hypothesized that disrupting TIM3 in NK cells using clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), would improve their anti-tumor effects in GBM, similarly to the blockade or disruption of immune checkpoints such as programmed death-1 (PD-1) [28] and lymphocyte activation gene 3 (LAG3) [26,29]. The gene discussed is LAG3; the disease is neoplasm.