The aim of this study was to evaluate the interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and matrix metalloproteinase 8 (MMP-8) levels in vaginally obtained amniotic fluid to investigate their prognostic value and determine the most appropriate cut-off values for the prediction of histological chorioamnionitis. The gene discussed is TNF; the disease is chorioamnionitis.