The study shows that radiotherapy, PLGA-R837@Cat and anti-CTLA-4 Ab, allowed for the best results also in the breast cancer orthotopic model (4T1), with superior efficacy with respect to PLGA-R837@Cat+X-ray, surgery+anti-CTLA-4 and surgery+PLGA-R837@Cat. The gene discussed is CTLA4; the disease is breast cancer.