EMD and infection: By using three different multiplicities of infection (MOI) with two different strains of HCMV (AD169 and TB40), a strong tendency of upregulation was found for these host factors in the period between 2–5 d p.i. Although some variation became evident in this measurement (e.g., for PKCα on d 5, Figure S1A, or emerin on d 2, Figure S1B), this increase in protein levels was mostly seen for the MOI of 0.1 and 0.5, whereas for a MOI of 2, the levels decreased in many cases, which may be due to the virus-induced cell lysis.