TFAM and obesity due to melanocortin 4 receptor deficiency: However, adipose-specific Tfam-deficient (aP2-Tfam KO) mice, generated by mating with aP2-Cre mice, developed lipoatrophy and reduced mitochondrial biogenesis but had resistance to diet-induced obesity (DIO), as well as improved glucose metabolism and fatty liver and increased energy expenditure [58].