The aim of this study was to investigate the possible cardioprotective effects of modulation of translocator protein (TSPO, also known as “peripheral benzodiazepine receptor”) with its ligand 4′-Chlorodiazepam (4′-ClDzp) in ISO-induced rat MI, alone or during the inhibition of nitric oxide synthesis by L-NAME. Here, TSPO is linked to myocardial infarction.