Considering that the detection 75% of the AA could be achieved using the three markers of the group B (i.e., CEACAM5, ITGA6 and MACC1), we then assayed various combinations of markers belonging to the group A in order to improve CRC detection using a maximum of 5 targets while keeping AA detection at 75% (Table 2). The gene discussed is MACC1; the disease is colorectal carcinoma.