In the study of Zeng and Briske-Anderson, the prolonged treatment of fibrosarcoma cells HT1080 with butyrate have shown the increased levels of MMP-2 and MMP-9 proteins but also increased the levels of tissue inhibitors of metalloproteinase-1 (TIMP), which altogether resulted in the decreased ability of these cells to create metastases [77]. This evidence concerns the gene TIMP1 and fibrosarcoma.