Here, we aimed to determine the eIF3A interactome by applying the BioID technique to two fast-growing cell lines, the human embryonic kidney cell line HEK-293T and the colon cancer cell line HCT-116, under optimal growth conditions and under severe stress caused by thapsigargin, an ER-stress-inducing compound. This evidence concerns the gene EIF3A and malignant colon neoplasm.