AKT1 and infection: To verify the experimental results, we also used an immunocytochemistry assay to observe the subcellular distribution of FOXO in hemocytes of shrimp after knockdown of Akt. The results showed that FOXO was significantly increased in the nucleus of hemocytes when Akt was knocked down 2 h post infection with V. anguillarum (Fig 5D and 5D’).