To explore whether the pathogen infection affects AKT activity, we detected the phosphorylation change of AKT after pathogen infection using a human p-AKT (Ser473) antibody (Phosphorylation site of shrimp AKT is Ser486), and found that the level of phosphorylated AKT decreased significantly in hemocytes and intestines of shrimp after 30 min of V. anguillarum infection (Fig 5E and 5F). Here, AKT1 is linked to infection.