The presence of HCC was confirmed on liver histology and by an increased expression of the HCC markers glypican-3 (Gpc3) and alpha-fetoprotein (Afp), although this did not reach significance for Gpc3 in the NASH-HCC model (Figure 1A, 1C, 1D and 1F). Here, GPC3 is linked to hepatocellular carcinoma.